5L8S
The crystal structure of a cold-adapted acylaminoacyl peptidase reveals a novel quaternary architecture based on the arm-exchange mechanism
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.93929 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 148.080, 151.100, 191.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.480 - 2.500 |
| R-factor | 0.21386 |
| Rwork | 0.212 |
| R-free | 0.24776 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ve6 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.282 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.740 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.128 | 0.399 |
| Number of reflections | 148344 | |
| <I/σ(I)> | 10.8 | 4.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.6 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 30% PEG 400, 0.1 M Na-Hepes (pH 7.5), and 0.2 M MgCl2 |
