5L8G
Crystal structure of Rhodospirillum rubrum Rru_A0973 mutant H65A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-02-16 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.7408 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 98.031, 120.294, 140.432 |
Unit cell angles | 90.00, 95.39, 90.00 |
Refinement procedure
Resolution | 48.860 - 2.974 |
R-factor | 0.1981 |
Rwork | 0.196 |
R-free | 0.24220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5da5 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.400 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.860 | 3.080 |
High resolution limit [Å] | 2.970 | 2.970 |
Rmerge | 0.177 | 0.863 |
Number of reflections | 133674 | |
<I/σ(I)> | 8.9 | 1.74 |
Completeness [%] | 100.0 | 99 |
Redundancy | 4.9 | 4.9 |
CC(1/2) | 0.989 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 1-2 ul protein mixed with 1 ul well solution over 1 ml well solution. 15% (w/v) PEG3350+ 15% calcium acetate |