5L8F
Crystal structure of Rhodospirillum rubrum Rru_A0973 mutant E32A, E62A, H65A.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-02-07 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.5 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 92.096, 92.096, 217.105 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.408 - 2.247 |
R-factor | 0.1822 |
Rwork | 0.179 |
R-free | 0.24000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5da5 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.085 |
Data reduction software | Aimless |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.410 | 2.330 |
High resolution limit [Å] | 2.247 | 2.247 |
Rmerge | 0.112 | 0.865 |
Number of reflections | 89902 | |
<I/σ(I)> | 11.79 | 1.72 |
Completeness [%] | 99.0 | 99 |
Redundancy | 5.8 | 5.3 |
CC(1/2) | 0.997 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 1-2 ul protein mixed with 1 ul well solution over 1 ml well solution. 20% PEG3350 + 0.2 M Magnesium formate |