5L8B
Crystal structure of Rhodospirillum rubrum Rru_A0973 mutant E62A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-04-27 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.7291 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 98.090, 120.228, 140.358 |
Unit cell angles | 90.00, 95.51, 90.00 |
Refinement procedure
Resolution | 48.870 - 2.210 |
R-factor | 0.1671 |
Rwork | 0.165 |
R-free | 0.21540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5da5 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.015 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.870 | 2.290 |
High resolution limit [Å] | 2.210 | 2.210 |
Rmerge | 0.098 | 1.009 |
Number of reflections | 133316 | |
<I/σ(I)> | 13.74 | 1.8 |
Completeness [%] | 100.0 | 97 |
Redundancy | 6.6 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 1-2 ul protein mixed with 1 ul well solution. 15% PEG3350 + 0.13 M CaAc |