5L88
AFAMIN ANTIBODY FRAGMENT, N14 FAB, L1- GLYCOSILATED, CRYSTAL FORM I, non-parsimonious model
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-06-29 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.782, 69.249, 87.803 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.380 - 1.880 |
R-factor | 0.17212 |
Rwork | 0.170 |
R-free | 0.21145 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1il1 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.377 |
Data reduction software | XDS |
Data scaling software | autoPROC |
Phasing software | BALBES |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.370 | 1.950 |
High resolution limit [Å] | 1.880 | 1.880 |
Rmerge | 0.076 | 0.800 |
Number of reflections | 34249 | |
<I/σ(I)> | 10 | 1.4 |
Completeness [%] | 99.4 | 97.7 |
Redundancy | 4.5 | 3.2 |
CC(1/2) | 0.998 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.8 | 291 | 200nL Fab 10mg/ml in 20 mM HEPES pH 7.5, 150 mM NaCl plus 200nL 30%w/v PEG 1k, 200mM KF, pH 5.8) |