5L81

Crystal structure of the PH domain of murine kindlin-3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-12-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.96861
Spacegroup name	P 21 21 2
Unit cell lengths	132.040, 36.190, 52.690
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.730 - 2.230
R-factor	0.2221
Rwork	0.219
R-free	0.27090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4bbk
RMSD bond length	0.007
RMSD bond angle	1.105
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	31.730
High resolution limit [Å]	2.100
Rmerge	0.121
Number of reflections	12441
<I/σ(I)>	13.68
Completeness [%]	96.0
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	4M sodium nitrate, 0.1M sodium acetate