5L7X
Afamin antibody fragment, N14 Fab, L1- glycosylated, crystal form II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-29 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.491, 72.196, 88.941 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.060 - 1.860 |
| R-factor | 0.19208 |
| Rwork | 0.190 |
| R-free | 0.22794 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1il1 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.501 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.470 | 1.930 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.074 | 0.934 |
| Number of reflections | 36491 | |
| <I/σ(I)> | 8.8 | 1 |
| Completeness [%] | 98.6 | 91.3 |
| Redundancy | 3.9 | 3 |
| CC(1/2) | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 200 nL Fab (10mg/ml in 20 mM HEPES pH 7.5, 150 mM NaCl) plus 200 nL cocktail (30% PEG 1k, 200mM Ammonium Fluoride, NH4F) |
