5L7O
X-ray structure of Triatoma virus empty capsid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | H 3 |
| Unit cell lengths | 305.586, 305.586, 796.486 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.910 - 3.600 |
| R-factor | 0.26874 |
| Rwork | 0.269 |
| R-free | 0.26917 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nap |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.159 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.910 | 3.700 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.162 | |
| Number of reflections | 315756 | |
| <I/σ(I)> | 5.17 | |
| Completeness [%] | 98.0 | 97 |
| Redundancy | 2.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291.15 | Crystal were growth at 291.15 K by vapor diffusion in sitting drop plates mixing equal volumes of the capsid solution at 3 mg/mL and of the reservoir solution: 100 mM Tris pH 8.5, 50 mM MgCl2 and 25% pentaerythritol propoxilate 629. |
