5L7L
Crystal Structure of Elp3 from Dehalococcoides mccartyi (390-407 GSGSG)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-12-13 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.033200 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 73.760, 161.410, 93.220 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.610 - 2.593 |
R-factor | 0.1836 |
Rwork | 0.182 |
R-free | 0.21870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | trunctaed 5L7J |
RMSD bond length | 0.004 |
RMSD bond angle | 0.684 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.660 |
High resolution limit [Å] | 2.590 | 2.590 |
Rmerge | 0.126 | 2.974 |
Number of reflections | 17592 | |
<I/σ(I)> | 14.8 | 0.94 |
Completeness [%] | 99.6 | 94.9 |
Redundancy | 13.06 | 11.8 |
CC(1/2) | 0.999 | 0.364 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 100 mM MES pH 6.3 and 4% PEG 4000 |