5L7K
The crystal structure of myristoylated NPHP3 peptide in complex with UNC119a
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-26 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97862 |
Spacegroup name | I 41 |
Unit cell lengths | 88.270, 88.270, 105.940 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.350 - 2.100 |
R-factor | 0.2188 |
Rwork | 0.216 |
R-free | 0.27820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3rbq |
RMSD bond length | 0.011 |
RMSD bond angle | 1.441 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.350 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.123 | 0.746 |
Number of reflections | 22466 | |
<I/σ(I)> | 13.47 | |
Completeness [%] | 99.9 | |
Redundancy | 11.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 1.75 M (NH4)2SO4, 0.1 M CAPS (pH 10.0) and 0.2 M Li2SO4 |