5L6Q
Refolded AL protein from cardiac amyloidosis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-12-01 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 58.020, 105.810, 34.750 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.400 |
R-factor | 0.14904 |
Rwork | 0.147 |
R-free | 0.18208 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4od2 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.668 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.500 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.054 | 0.525 |
Number of reflections | 42100 | |
<I/σ(I)> | 12.6 | 2.6 |
Completeness [%] | 97.6 | 98.7 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M MES, 18% PEG 8000, 0.1 M Zinc acetate |