5L6M
Structure of Caulobacter crescentus VapBC1 (VapB1deltaC:VapC1 form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-17 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.976 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 94.400, 63.600, 184.700 |
| Unit cell angles | 90.00, 94.10, 90.00 |
Refinement procedure
| Resolution | 46.600 - 1.900 |
| R-factor | 0.198 |
| Rwork | 0.196 |
| R-free | 0.23960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5k8j |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.917 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2152: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.600 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 85224 | |
| <I/σ(I)> | 10.9 | 1.5 |
| Completeness [%] | 98.8 | 98.9 |
| Redundancy | 6.7 | 6.6 |
| CC(1/2) | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 292.15 | 0.1 M Sodium malonate pH 4.0, 12% (w/v) PEG 3350, 5 mM MgCl2, 5 mM beta-mercaptoethanol, 25% glycerol |
