5L6L
Structure of Caulobacter crescentus VapBC1 bound to operator DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-3 |
Synchrotron site | MAX II |
Beamline | I911-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-07 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.972 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 110.180, 144.160, 160.540 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.800 - 2.700 |
R-factor | 0.1811 |
Rwork | 0.178 |
R-free | 0.24050 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5k8j |
RMSD bond length | 0.009 |
RMSD bond angle | 1.108 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2152: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.800 | 2.770 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 67883 | |
<I/σ(I)> | 11.7 | 1.24 |
Completeness [%] | 100.0 | 100 |
Redundancy | 15.7 | 15.7 |
CC(1/2) | 0.992 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 292.15 | 20% PEG 3350, 0.2 Succinic acid pH 7, 5 mM MgCl2, 5 mM beta-mercapo ethanol, 20% glycerol |