5L6G
Xylooligosaccharide oxidase from Myceliophthora thermophila C1 in complex with Xylose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR-H |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-09-07 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 123.300, 59.353, 68.456 |
| Unit cell angles | 90.00, 90.99, 90.00 |
Refinement procedure
| Resolution | 53.480 - 1.790 |
| R-factor | 0.17485 |
| Rwork | 0.173 |
| R-free | 0.20706 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 5k8e |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.394 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.480 | 1.820 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.071 | 0.343 |
| Number of reflections | 46206 | |
| <I/σ(I)> | 11 | 2.6 |
| Completeness [%] | 98.3 | 78.6 |
| Redundancy | 3.8 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 294 | 15-18% PEG3350, 0.1 M MES buffer, 0.2 M ammonium chloride |
