5L6F
Xylooligosaccharide oxidase from Myceliophthora thermophila C1 in complex with Xylobiose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR-H |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-09-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 123.591, 59.226, 68.502 |
| Unit cell angles | 90.00, 90.76, 90.00 |
Refinement procedure
| Resolution | 53.400 - 1.800 |
| R-factor | 0.15379 |
| Rwork | 0.152 |
| R-free | 0.18371 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 5k8e |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.454 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.400 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.066 | 0.274 |
| Number of reflections | 45931 | |
| <I/σ(I)> | 11.4 | 3.3 |
| Completeness [%] | 99.4 | 90 |
| Redundancy | 3.6 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 294 | 15-18% PEG3350, 0.1 M MES buffer, 0.2 M ammonium chloride |
