5L5K
Plexin A4 full extracellular region, domains 1 to 10, data to 7.5 angstrom, spacegroup P4(1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-12-12 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.8726 |
Spacegroup name | P 41 |
Unit cell lengths | 220.593, 220.593, 65.938 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 55.148 - 7.501 |
R-factor | 0.3537 |
Rwork | 0.353 |
R-free | 0.37140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5l56 |
RMSD bond length | 0.019 |
RMSD bond angle | 2.439 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.000 | |
High resolution limit [Å] | 7.500 | 7.500 |
Rmerge | 0.207 | 1.210 |
Number of reflections | 4253 | |
<I/σ(I)> | 12.2 | |
Completeness [%] | 99.8 | |
Redundancy | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293.5 | 29% (v/v) glycerol, 4.3% (w/v) PEG 8k, 53 mM TRIS hydrochloride, pH 8.5 |