5L5G
Plexin A2 full extracellular region, domains 1 to 8 modeled, data to 10 angstrom
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-02-26 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97922 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 238.400, 238.400, 642.180 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 63.055 - 10.000 |
| R-factor | 0.3368 |
| Rwork | 0.335 |
| R-free | 0.37020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3okt |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.728 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.055 | |
| High resolution limit [Å] | 10.000 | 10.000 |
| Rmerge | 0.187 | 0.882 |
| Number of reflections | 11079 | |
| <I/σ(I)> | 5.1 | |
| Completeness [%] | 93.9 | |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293.5 | 0.68 M potassium/sodium tartrate, 85 mM HEPES, pH 7.5 |
