5L56
Plexin A1 full extracellular region, domains 1 to 10, to 4 angstrom
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-04-29 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.96131 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 195.343, 195.343, 176.180 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 87.510 - 4.000 |
| R-factor | 0.259 |
| Rwork | 0.257 |
| R-free | 0.30800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3okt |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.243 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.000 | |
| High resolution limit [Å] | 4.000 | 4.000 |
| Rmerge | 0.241 | 1.040 |
| Number of reflections | 28954 | |
| <I/σ(I)> | 5.2 | |
| Completeness [%] | 99.1 | |
| Redundancy | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293.5 | 6% (w/v) PEG 4k, 5 mM tricine or 5 mM TRIS |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293.5 | 6% (w/v) PEG 4k, 5 mM tricine or 5 mM TRIS |
