5L4O
Structure of an E.coli initiator tRNAfMet A1-U72 variant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-04-07 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.978570 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 35.150, 142.620, 202.120 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.167 - 2.799 |
R-factor | 0.1982 |
Rwork | 0.197 |
R-free | 0.22650 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3cw5 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.140 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.230 | 2.950 |
High resolution limit [Å] | 2.790 | 2.790 |
Rmerge | 0.084 | 2.464 |
Number of reflections | 13192 | |
<I/σ(I)> | 17.03 | 0.95 |
Completeness [%] | 99.2 | 95.5 |
Redundancy | 9.84 | 9.74 |
CC(1/2) | 0.999 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 20% PEG 6000, 2M NaCl |