5L3X
Crystal structure of negative elongation factor subcomplex NELF-AC
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-04-29 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.0 |
Spacegroup name | I 21 3 |
Unit cell lengths | 185.070, 185.070, 185.070 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.267 - 2.750 |
R-factor | 0.2378 |
Rwork | 0.237 |
R-free | 0.25580 |
Structure solution method | SIRAS |
RMSD bond length | 0.003 |
RMSD bond angle | 0.662 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELXDE |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.267 | 2.820 |
High resolution limit [Å] | 2.750 | 2.750 |
Number of reflections | 27492 | |
<I/σ(I)> | 32.2 | 1.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 39.8 | 40.7 |
CC(1/2) | 1.000 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 14-14.5% (w/v) PEG 3350 200mM sodium malonate pH 6.8-7.0 |