5L3W

Structure of the crenarchaeal FtsY GTPase bound to GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-06-25
Detector	ADSC QUANTUM 210
Wavelength(s)	0.934
Spacegroup name	H 3
Unit cell lengths	144.906, 144.906, 49.723
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.831 - 2.400
R-factor	0.1755
Rwork	0.171
R-free	0.21750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5L3S chain B
RMSD bond length	0.008
RMSD bond angle	0.907
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.830	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	15249
<I/σ(I)>	14.9
Completeness [%]	100.0
Redundancy	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M CHES pH 9.0, 10 % (w/v) PEG 8000