5L3D
Human LSD1/CoREST: LSD1 Y761H mutation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-02-15 |
Detector | DECTRIS PILATUS 300K |
Wavelength(s) | 1 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 120.309, 177.687, 235.233 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 100.000 - 2.600 |
R-factor | 0.20781 |
Rwork | 0.208 |
R-free | 0.21877 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.008 |
RMSD bond angle | 1.218 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.650 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.970 | |
Number of reflections | 76680 | |
<I/σ(I)> | 10.5 | 1 |
Completeness [%] | 99.2 | 100 |
Redundancy | 4.4 | 4.5 |
CC(1/2) | 0.992 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | Tartrate 1-1.4 M, ADA 100 mM pH 5.6 |