5L3B

Human LSD1/CoREST: LSD1 D556G mutation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-06
Detector	DECTRIS PILATUS 300K
Wavelength(s)	0.97626
Spacegroup name	I 2 2 2
Unit cell lengths	119.088, 179.122, 234.411
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 3.300
R-factor	0.19483
Rwork	0.195
R-free	0.20783
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	2v1d
RMSD bond length	0.008
RMSD bond angle	1.308
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.450
High resolution limit [Å]	3.300	3.300
Rmerge	0.131	0.613
Number of reflections	37869
<I/σ(I)>	7.2	1.7
Completeness [%]	99.6	99.6
Redundancy	3.8	3.9
CC(1/2)	0.988

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	Tartrate 1-1.4 M, ADA 100 mM pH 6.5