5L39

The structure of the fused permuted hexameric shell protein MSM0275 from the RMM microcompartment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-16
Detector	RAYONIX MX-300
Wavelength(s)	0.97949
Spacegroup name	C 2 2 21
Unit cell lengths	132.140, 143.480, 116.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.600 - 2.100
R-factor	0.1838
Rwork	0.182
R-free	0.22390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ht5
RMSD bond length	0.005
RMSD bond angle	0.872
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.600	2.150
High resolution limit [Å]	2.100	2.100
Rmerge		0.897
Number of reflections	64985
<I/σ(I)>	11.96	2.4
Completeness [%]	100.0	100
Redundancy	7.5	7.5
CC(1/2)	0.997	0.834

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	293	20 mg/mL protein, 0.5 M Na citrate, 20% PEG3350