5L38
The structure of the hexagonal shell protein MSM0272 from the RMM microcompartment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-03-16 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97949 |
Spacegroup name | P 43 |
Unit cell lengths | 93.600, 93.600, 192.540 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.135 - 2.200 |
R-factor | 0.2017 |
Rwork | 0.200 |
R-free | 0.23320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2qw7 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.684 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.260 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.745 | |
Number of reflections | 83627 | |
<I/σ(I)> | 18.4 | 2.5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 5.1 | 5.1 |
CC(1/2) | 0.999 | 0.640 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M Tris, 11% ethylene glycol, 10 % PEG 8000, 7mg/ml protein |