5L31
Crystal structure of an engineered metal-free RIDC1 variant containing five disulfide bonds.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-04-07 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.594, 77.534, 88.063 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.770 - 2.400 |
R-factor | 0.22716 |
Rwork | 0.225 |
R-free | 0.26701 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4jea |
RMSD bond length | 0.008 |
RMSD bond angle | 1.302 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.770 | 2.530 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.230 | |
Number of reflections | 17325 | |
<I/σ(I)> | 14.9 | 5.7 |
Completeness [%] | 99.8 | 99.4 |
Redundancy | 6.9 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | Drop consists of 1 uL of 45% MPD, and 0.1 M Bis Tris (pH 6.5) mixed with 1.5 uL of 2.8 mM protein |