5L2V
Catalytic domain of LPMO Lmo2467 from Listeria monocytogenes
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-07-28 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | .978 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 61.575, 139.712, 90.591 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.150 - 1.100 |
R-factor | 0.129 |
Rwork | 0.129 |
R-free | 0.14500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bem |
RMSD bond length | 0.008 |
RMSD bond angle | 1.428 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.120 |
High resolution limit [Å] | 1.100 | 1.100 |
Rmerge | 0.079 | 0.749 |
Number of reflections | 148923 | |
<I/σ(I)> | 21.6 | 1.6 |
Completeness [%] | 94.8 | 66.9 |
Redundancy | 6.7 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 297 | Protein: 8.0 mg/ml, 0.01 M Tris HCl (pH 8.3); Screen: JCSG+ H9 (Qiagen),0.2 M AmSO4, 0.1 M Bis-Tris (pH 5.5), 25% (w/v) PEG3350. |