5L2U
Oligomer crystal structure of CC chemokine 5 (CCL5)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-07-21 |
Detector | DECTRIS PILATUS3 X 6M |
Wavelength(s) | 0.97926 |
Spacegroup name | C 2 2 2 |
Unit cell lengths | 121.799, 210.082, 126.718 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.648 - 2.280 |
R-factor | 0.1793 |
Rwork | 0.178 |
R-free | 0.20910 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5cmd |
RMSD bond length | 0.008 |
RMSD bond angle | 0.881 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 48.648 |
High resolution limit [Å] | 2.280 |
Number of reflections | 76849 |
<I/σ(I)> | 33.74 |
Completeness [%] | 99.8 |
Redundancy | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 303.15 | 0.1 HEPES, 300 mM NaCl, 3.5% 2-propanol |