5L2L
Nab2 Zn fingers 5-7 bound to A11G RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-01-18 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9282 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.669, 85.035, 64.956 |
| Unit cell angles | 90.00, 99.07, 90.00 |
Refinement procedure
| Resolution | 46.085 - 1.550 |
| R-factor | 0.1319 |
| Rwork | 0.131 |
| R-free | 0.15160 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.944 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.085 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.043 | 0.689 |
| Number of reflections | 72125 | |
| <I/σ(I)> | 20.8 | 1.9 |
| Completeness [%] | 99.4 | 98.9 |
| Redundancy | 6.4 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 291 | 30% ethanol, PEG6000, ammonium acetate, Tris buffer, pH 7.4. See publication for details. |
