5L2B
Structure of CNTnw N149S, E332A in an outward-facing state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-07 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97910 |
Spacegroup name | P 61 |
Unit cell lengths | 121.040, 121.040, 277.440 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 98.058 - 3.800 |
R-factor | 0.2533 |
Rwork | 0.251 |
R-free | 0.28950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5l26 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.724 |
Data reduction software | iMOSFLM (7.2.1) |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 98.060 | 4.100 |
High resolution limit [Å] | 3.800 | 3.800 |
Rpim | 0.080 | 0.540 |
Number of reflections | 22489 | |
<I/σ(I)> | 6.2 | 2 |
Completeness [%] | 99.4 | 99.8 |
Redundancy | 4.3 | 4.3 |
CC(1/2) | 0.990 | 0.540 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 290 | 200 mM choline chloride, 14% Poly(ethylene glycol) monomethyl ether 2000 |