5L23
Crystal structure of the complex between the N-terminal SH3 domain of CrkII and a proline-rich ligand
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 113 |
Detector technology | IMAGE PLATE |
Collection date | 2015-05-01 |
Detector | RIGAKU |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 27.600, 41.340, 56.820 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.430 - 1.770 |
R-factor | 0.17587 |
Rwork | 0.174 |
R-free | 0.21037 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1cka |
RMSD bond length | 0.029 |
RMSD bond angle | 2.611 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.430 | 1.870 |
High resolution limit [Å] | 1.770 | 1.770 |
Rmerge | 0.160 | 0.200 |
Number of reflections | 6759 | |
<I/σ(I)> | 11.7 | 8.4 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 7.4 | 7.2 |
CC(1/2) | 0.977 | 0.960 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 286 | Lithium sulfate monohydrate, PEG 3350 |