5L1F

AMPA subtype ionotropic glutamate receptor GluA2 in complex with noncompetitive inhibitor Perampanel

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-11
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	91.740, 109.345, 594.835
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.570 - 4.000
R-factor	0.2388
Rwork	0.237
R-free	0.27210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kg2
RMSD bond length	0.002
RMSD bond angle	0.497
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.570	4.140
High resolution limit [Å]	4.000	4.000
Rmerge	0.090	0.900
Number of reflections	51631
<I/σ(I)>	8.15
Completeness [%]	99.5	97.7
Redundancy	6.45	5.55
CC(1/2)	0.998	0.490

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	11-14% (w/v) PEG 6,000, 0.1 M ammonium phosphate and 0.1 M TRIS (pH 7.9-8.0)