5L1A

Crystal structure of uncharacterized protein LPG2271 from Legionella pneumophila

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2013-08-15
Detector	ADSC QUANTUM 210r
Wavelength(s)	1.7712
Spacegroup name	P 21 21 21
Unit cell lengths	41.154, 44.657, 54.136
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.449 - 2.400
R-factor	0.1937
Rwork	0.192
R-free	0.24640
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.515
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	HKL-3000
Refinement software	PHENIX (dev_2386)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.440
High resolution limit [Å]	2.400	6.510	2.400
Rmerge	0.097	0.068	0.196
Total number of observations	24051
Number of reflections	4158
<I/σ(I)>	15.6
Completeness [%]	98.1	98.4	80.9
Redundancy	5.8	5.5	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.5	298	0.1M Citric acid, 25% PEG3350