5L16
Crystal Structure of N-terminus truncated selenophosphate synthetase from Leishmania major
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-05-24 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.9200 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 55.950, 81.690, 177.920 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.845 - 1.882 |
R-factor | 0.1753 |
Rwork | 0.173 |
R-free | 0.21310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3fd5 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.018 |
Data scaling software | SCALA (3.3.16) |
Phasing software | PHASER |
Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 88.960 | 40.973 | 1.980 |
High resolution limit [Å] | 1.882 | 5.950 | 1.882 |
Rmerge | 0.032 | 0.687 | |
Number of reflections | 33416 | ||
<I/σ(I)> | 11.4 | 18.1 | 1 |
Completeness [%] | 99.7 | 98.8 | 99.9 |
Redundancy | 5 | 5.3 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 291 | 100mM tri-sodium ciitrate pH 5.2, 200mM ammonium sulphate, 21% PEG4000. |