5L12
Crystal structure of 2C-methyl-D-erythritol 2,4-clycodiphosphate synthase from BURKHOLDERIA PSEUDOMALLEI double mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2015-03-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 116.859, 68.140, 60.408 |
| Unit cell angles | 90.00, 96.57, 90.00 |
Refinement procedure
| Resolution | 34.070 - 1.716 |
| R-factor | 0.1663 |
| Rwork | 0.165 |
| R-free | 0.20360 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.249 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 34.070 |
| High resolution limit [Å] | 1.716 |
| Number of reflections | 50230 |
| <I/σ(I)> | 18.1 |
| Completeness [%] | 100.0 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 292 | Tris, NaCl, PEG 4000, ZnCl2 |
