5L0Z

Crystal Structure of AdoMet bound rRNA methyltransferase from Sinorhizobium meliloti

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-08-20
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.542
Spacegroup name	P 21 21 21
Unit cell lengths	64.855, 82.896, 108.806
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.940 - 2.900
R-factor	0.19437
Rwork	0.191
R-free	0.26498
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.667
Data scaling software	HKL-3000
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall
Low resolution limit [Å]	65.940
High resolution limit [Å]	2.900
Number of reflections	13571
<I/σ(I)>	28.51
Completeness [%]	100.0
Redundancy	6.7
CC(1/2)	0.860

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	300	5mM Cobalt chloride , 5mM Magnesium chloride hexahydrate, 0.1M HEPES pH 7.5, 9% wt/vol polyethylene glycol 3,350