5L0Y

Crystal Structure of a Sec72-ssa1 c-terminal peptide fusion protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-16
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.0332
Spacegroup name	P 21 21 2
Unit cell lengths	76.504, 118.535, 164.049
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	96.080 - 2.870
R-factor	0.238
Rwork	0.236
R-free	0.28300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5l0w
RMSD bond length	0.007
RMSD bond angle	1.060
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	164.050
High resolution limit [Å]	2.870
Number of reflections	34691
<I/σ(I)>	16
Completeness [%]	99.3
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.1 M ammonium citrate pH 7.0, 12% PEG 3350