5L0W
Structure of post-translational translocation Sec71/Sec72 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-10 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 108.600, 108.600, 271.170 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.100 - 3.035 |
| R-factor | 0.2242 |
| Rwork | 0.223 |
| R-free | 0.25020 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.911 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.100 | 3.130 |
| High resolution limit [Å] | 3.035 | 3.035 |
| Rmerge | 0.137 | 1.700 |
| Number of reflections | 18882 | |
| <I/σ(I)> | 17 | |
| Completeness [%] | 99.8 | 99 |
| Redundancy | 9.4 | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 1.4M ammonium tatrate dibasic, 0.1M Tris/HCl pH 8.5 |
