5L0L
Crystal structure of Uncharacterized protein LPG0439
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 129.688, 129.688, 117.143 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.010 - 1.800 |
| R-factor | 0.17 |
| Rwork | 0.169 |
| R-free | 0.18890 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.529 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.820 |
| High resolution limit [Å] | 1.800 | 6.150 | 1.800 |
| Rmerge | 0.125 | 0.078 | 0.907 |
| Total number of observations | 630221 | ||
| Number of reflections | 54185 | ||
| <I/σ(I)> | 7.9 | ||
| Completeness [%] | 99.9 | 98.2 | 100 |
| Redundancy | 11.6 | 10.6 | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 297 | % Peg2KMME, Ammonium sulfate 0.2M, 0.1M sodium acetate |
