5L0F

Human metavinculin quadruple mutant (residues 959-1134)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-23
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	47.153, 70.909, 118.060
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.790 - 2.760
R-factor	0.2038
Rwork	0.201
R-free	0.24750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3myi
RMSD bond length	0.010
RMSD bond angle	1.070
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	BUSTER (2.10.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.790	2.910
High resolution limit [Å]	2.760	2.760
Rpim	0.022
Number of reflections	10731
<I/σ(I)>	28.1	13
Completeness [%]	99.8	99.7
Redundancy	9.6	9.1
CC(1/2)	0.999	0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.8	294	20 mM citric acid, 80 mM Bis-Tris propane (pH 8.8), and 14 to 16% (w/v) PEG 3350