5L0F
Human metavinculin quadruple mutant (residues 959-1134)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-07-23 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.153, 70.909, 118.060 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 60.790 - 2.760 |
R-factor | 0.2038 |
Rwork | 0.201 |
R-free | 0.24750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3myi |
RMSD bond length | 0.010 |
RMSD bond angle | 1.070 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | BUSTER (2.10.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.790 | 2.910 |
High resolution limit [Å] | 2.760 | 2.760 |
Rpim | 0.022 | |
Number of reflections | 10731 | |
<I/σ(I)> | 28.1 | 13 |
Completeness [%] | 99.8 | 99.7 |
Redundancy | 9.6 | 9.1 |
CC(1/2) | 0.999 | 0.995 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 294 | 20 mM citric acid, 80 mM Bis-Tris propane (pH 8.8), and 14 to 16% (w/v) PEG 3350 |