5L07
Crystal Structure of Quorum-Sensing Transcriptional Activator from Yersinia enterocolitica
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-04-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97918 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 64.371, 123.399, 106.907 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.661 - 2.200 |
R-factor | 0.1748 |
Rwork | 0.173 |
R-free | 0.21420 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 0.892 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX ((1.10pre_2104: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.703 | |
Number of reflections | 21587 | |
<I/σ(I)> | 17.1 | 3.07 |
Completeness [%] | 97.3 | 95.8 |
Redundancy | 5.5 | 4.9 |
CC(1/2) | 0.824 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 289 | 0.1 M sodium acetate pH 4.5, 2.5 M ammonium sulfate |