5L00

Self-complimentary RNA 15mer binding with GMP monomers

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	90
Detector technology	CCD
Collection date	2016-05-19
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 3
Unit cell lengths	43.846, 43.846, 86.450
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.972 - 1.250
R-factor	0.20999
Rwork	0.209
R-free	0.23859
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nfo
RMSD bond length	0.030
RMSD bond angle	2.985
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.290
High resolution limit [Å]	1.250	1.250
Rmerge	0.044	0.277
Number of reflections	51401
<I/σ(I)>	34.4	2.46
Completeness [%]	92.7	64.5
Redundancy	5	2.3
CC(1/2)	0.956	0.854

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	10% v/v (+/-)-2-Methyl-2,4-pentanediol, 0.040 M Sodium cacodylate trihydrate pH 7.0, 0.012 M Spermine tetrahydrochloride, 0.012 M Sodium chloride, 0.080 M Potassium chloride