5L00
Self-complimentary RNA 15mer binding with GMP monomers
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2016-05-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | P 3 |
Unit cell lengths | 43.846, 43.846, 86.450 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.972 - 1.250 |
R-factor | 0.20999 |
Rwork | 0.209 |
R-free | 0.23859 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4nfo |
RMSD bond length | 0.030 |
RMSD bond angle | 2.985 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.290 |
High resolution limit [Å] | 1.250 | 1.250 |
Rmerge | 0.044 | 0.277 |
Number of reflections | 51401 | |
<I/σ(I)> | 34.4 | 2.46 |
Completeness [%] | 92.7 | 64.5 |
Redundancy | 5 | 2.3 |
CC(1/2) | 0.956 | 0.854 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 10% v/v (+/-)-2-Methyl-2,4-pentanediol, 0.040 M Sodium cacodylate trihydrate pH 7.0, 0.012 M Spermine tetrahydrochloride, 0.012 M Sodium chloride, 0.080 M Potassium chloride |