5KZW
Crystal structure of human GAA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-07 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.18076 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 97.110, 102.590, 128.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 80.310 - 2.000 |
| R-factor | 0.156 |
| Rwork | 0.154 |
| R-free | 0.18500 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 5KZR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.469 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless (0.5.12) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.310 | 2.040 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.083 | 0.416 |
| Number of reflections | 73011 | |
| <I/σ(I)> | 13.7 | |
| Completeness [%] | 84.0 | 93.3 |
| Redundancy | 4.5 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 sodium HEPES, 2% (v/v) PEG 400, 2M ammonium sulfate |
