5KZN

Metabotropic Glutamate Receptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2008-02-21
Detector	RAYONIX MX-225
Wavelength(s)	0.9793
Spacegroup name	P 65 2 2
Unit cell lengths	129.850, 129.850, 252.483
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.170 - 2.800
R-factor	0.2216
Rwork	0.220
R-free	0.24910
RMSD bond length	0.010
RMSD bond angle	1.170
Data reduction software	d*TREK
Data scaling software	SCALA (3.3.22)
Refinement software	BUSTER (2.11.6)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	25.170	46.753	2.950
High resolution limit [Å]	2.800	8.850	2.800
Rmerge		0.046	0.840
<I/σ(I)>		13.7	0.9
Completeness [%]		99.2	100
Redundancy		16.9	19.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	100mM Tris HCl pH 8.5,.5% PEG MME 5K, 800mM Potassium Sodium Tartrate