5KZM
Crystal structure of Tryptophan synthase alpha-beta chain complex from Francisella tularensis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-02-16 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97934 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 111.176, 171.986, 76.109 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.496 - 2.804 |
R-factor | 0.1858 |
Rwork | 0.183 |
R-free | 0.23470 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.574 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX (dev_2386) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.850 |
High resolution limit [Å] | 2.800 | 7.560 | 2.800 |
Rmerge | 0.132 | 0.057 | 0.981 |
Rmeas | 0.143 | 0.064 | |
Rpim | 0.067 | 0.030 | 0.491 |
Total number of observations | 87874 | ||
Number of reflections | 18264 | ||
<I/σ(I)> | 5.8 | ||
Completeness [%] | 100.0 | 99.8 | 100 |
Redundancy | 4.8 | 4.4 | 4.9 |
CC(1/2) | 0.995 | 0.566 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2M calcium acetate, 0.1M imidazole, 10% PEG 8000 |