5KZJ
Loop Deletion mutant of Paracoccus denitrificans AztC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-05-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.09998 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.826, 104.314, 64.320 |
| Unit cell angles | 90.00, 110.70, 90.00 |
Refinement procedure
| Resolution | 46.470 - 2.200 |
| R-factor | 0.16096 |
| Rwork | 0.159 |
| R-free | 0.20009 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4XRV |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.910 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.066 | 0.461 |
| Number of reflections | 38783 | |
| <I/σ(I)> | 18.618 | 2.312 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 4.1 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 7 | 292 | 4.0 - 5.0 M sodium formate, 0.1 M bis-tris propane |
