5KZA

Crystal structure of the Rous sarcoma virus matrix protein (aa 2-102). Space group I41

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	2007-10-16
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.54179
Spacegroup name	I 41
Unit cell lengths	79.000, 79.000, 27.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.500 - 1.860
R-factor	0.16614
Rwork	0.164
R-free	0.20991
RMSD bond length	0.011
RMSD bond angle	1.392
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall
Low resolution limit [Å]	39.500
High resolution limit [Å]	1.860
Rmerge	0.055
Number of reflections	7363
<I/σ(I)>	56
Completeness [%]	99.5
Redundancy	12.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	291.15	18%(w/v) PEG 8000, 0.2 M Succinic acid/KOH pH 5.5, 1.0 M Ammonium nitrate