5KZ9
Crystal structure of the Rous sarcoma virus matrix protein.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-02 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0332 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 66.200, 66.200, 218.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.000 - 2.850 |
| R-factor | 0.19761 |
| Rwork | 0.195 |
| R-free | 0.23855 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.325 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 49.000 |
| High resolution limit [Å] | 2.850 |
| Rmerge | 0.070 |
| Number of reflections | 6104 |
| <I/σ(I)> | 34 |
| Completeness [%] | 99.9 |
| Redundancy | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 10.3 | 293 | 2.60 M Ammonium formate, 0.20 M Beta-Alanine/KOH pH 10.3 |
