5KYY

Crystal structure of Sec23 and TANGO1 peptide4 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-11
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	101.386, 139.056, 149.444
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.006 - 3.403
R-factor	0.1829
Rwork	0.177
R-free	0.25250
RMSD bond length	0.010
RMSD bond angle	1.367
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.300
Number of reflections	24866
<I/σ(I)>	13.7
Completeness [%]	99.8
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	100 mM HEPES pH 7.5, 5.5 %(w/v) PEG 4000, 100 mM MgSO4