5KYT
Structure of Photinus pyralis Luciferase red light emitting variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2008-01-08 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9179 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 92.587, 92.587, 294.465 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.452 - 2.001 |
| R-factor | 0.1803 |
| Rwork | 0.178 |
| R-free | 0.21830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4g36 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.051 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.452 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.093 | 0.296 |
| Number of reflections | 93124 | |
| <I/σ(I)> | 16.8 | 1.9 |
| Completeness [%] | 93.1 | 64.3 |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 23 % PEG 8000, 300 mM Na/K tartrate, 1.00 mM Tris |
